Escherichia coli remains at the forefront of recombinant protein production, owing to its rapid growth, well-characterised genetics and the ease with which it can be genetically manipulated. The ...

Immobilized metal affinity chromatography (IMAC) is a reliable method for isolation of recombinant protein following over-expression in Escherichia coli. The polyhistidine tag (6–10 consecutive amino ...

By reconstructing a non-natural metabolic route in Escherichia coli, the researchers demonstrated that simple sugars can be ...